Composite

Part:BBa_K5241008:Design

Designed by: yingmei tang   Group: iGEM24_ECIB-PKU   (2024-09-25)


pPICZaAPkrhdd-6xH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 5174
    Illegal PstI site found at 925
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal NheI site found at 4469
    Illegal PstI site found at 925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal BglII site found at 1089
    Illegal BglII site found at 2620
    Illegal BamHI site found at 2977
    Illegal XhoI site found at 1075
    Illegal XhoI site found at 2284
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 5174
    Illegal PstI site found at 925
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 43
    Illegal EcoRI site found at 52
    Illegal EcoRI site found at 967
    Illegal XbaI site found at 5174
    Illegal PstI site found at 925
    Illegal NgoMIV site found at 342
    Illegal AgeI site found at 319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5330
    Illegal BsaI.rc site found at 316
    Illegal BsaI.rc site found at 3129


Design Notes

none


Source

Pseudomonas_koreensis

References